浩瀚色谱采用毛细管柱气相色谱法测定植物油中脂肪酸组成。实验中选用氢氧化钾-甲醇溶液作为甲酯化试剂对样品进行处理,再用正己烷提取,通过气相色谱仪对脂肪酸组成进行分离和鉴定。总共分离出15种脂肪酸,结果表明:植物油中的脂肪酸以棕榈酸、硬脂酸、油酸、亚油酸为主;只有胡麻油中油酸和亚油酸相对含量为31.7%,其他四种植物油油酸和亚油酸含量均高于75%;而菜籽油中的油酸相对含量最高,大豆油中的亚油酸相对含量最高;五种植物油中不饱和脂肪酸含量高于饱和脂肪酸含量,其中菜籽油中不饱和脂肪酸相对含量最高。

　　名称:毛细管柱

　　型号:HH-PEG-20M

　　规格:30m*0.32mm*0.25um

　　*高温度:250°C

　　编号:20240514009

　　应用:植物油在脂肪酸的测定 气相色谱法

　　适用于安捷伦6890,7890,7820,8860,8890；岛津GC-2014，GC-2018，GC-2030，GC-2010

　　Haohan chromatography uses capillary column gas chromatography to determine the fatty acid composition in vegetable oil. In the experiment, potassium hydroxide methanol solution was selected as the methylation reagent to treat the sample, followed by n-hexane extraction. The fatty acid composition was separated and identified by gas chromatography. A total of 15 fatty acids were isolated, and the results showed that the main fatty acids in vegetable oil were palmitic acid, stearic acid, oleic acid, and linoleic acid; Only the relative content of oleic acid and linoleic acid in sesame oil is 31.7%, while the content of oleic acid and linoleic acid in the other four plant oils is higher than 75%; The relative content of oleic acid in rapeseed oil is the highest, while the relative content of linoleic acid in soybean oil is the highest; The content of unsaturated fatty acids in five types of vegetable oils is higher than that of saturated fatty acids, with rapeseed oil having the highest relative content of unsaturated fatty acids.

　　Name: Capillary Column

　　Model: HH-PEG-20M

　　Specification: 30m * 0.32mm * 0.25um

　　*High temperature: 250 ° C

　　Number: 20240514009

　　Application: Vegetable oils - Determination of fatty acids - Gas chromatography method

　　Applicable to Agilent 68907890782088608890; Shimadzu GC-2014, GC-2018, GC-2030, GC-2010