食品中的维生素D_2含量很低,即使是强化食品每百克中也只含维生素D_2100～400IU(2.5～10μg),所以检测食品中的维生素D_2一直被认为是非常困难的。长期以来人们一直在努力寻求检测食品中维生素D_2的有效方法。六十年代曾采用《生物学试验法》,但此法费用高,操作复杂,误差大,目前仅用于鉴定新型维生素D衍生物的生理效力。

　　维生素D在动物骨骼组织矿化代谢过程中起着十分重要的作用,它不仅促进钙与磷在肠道的吸收,而且也作用于骨骼组织,促进钙磷最终形成骨质的基本结构,当维生素D缺乏时会导致动物患佝偻病和软骨病等。饲料预混剂中通常加入适量维生素D等作为营养强化剂,其含量的多少直接影响着预混剂的质量。近年来随着高效液相色谱技术的快速发展,已被广泛应用于食品及饲料成分分析领域。

　　对于高效液相色谱分析预混剂中的维生素D，浩瀚色谱就测定过程中的提取(皂化)探索了方法的准确度,就精密度做了重现性研究,建立了一种快速准确的测定方法。

　　名称:液相硅胶柱

　　规格:250MMX4.6MMX5uM

　　货号:20240510003

　　应用:

　　GB/T 17818-1999 饲料中维生素D3的测定 高xiao液相色谱法

　　GB/T 17818-1999 饲料中维生素D3的测定 gao效液相色谱法

　　GB5009.296-2023食品安quan国jia标准 食品中维生素D的测定

　　The content of vitamin D2 in food is very low, and even fortified foods only contain 100-400 IU (2.5-10 IU) of vitamin D2 per hundred grams μ g) Therefore, detecting vitamin D2 in food has always been considered very difficult. For a long time, people have been striving to find effective methods for detecting vitamin D2 in food. In the 1960s, the Biological Experiment Method was used, but this method is expensive, complex to operate, and has large errors. Currently, it is only used to identify the physiological efficacy of new vitamin D derivatives.

　　Vitamin D plays a crucial role in the mineralization and metabolism of animal bone tissue. It not only promotes the absorption of calcium and phosphorus in the intestine, but also acts on bone tissue, promoting the formation of calcium and phosphorus into the basic structure of bone. When vitamin D is deficient, it can lead to diseases such as rickets and chondropathy in animals. An appropriate amount of vitamin D and other nutrients are usually added to feed premixes as nutritional enhancers, and their content directly affects the quality of the premix. In recent years, with the rapid development of high-performance liquid chromatography technology, it has been widely used in the field of food and feed ingredient analysis.

　　For the analysis of vitamin D in premixes by high-performance liquid chromatography, Haohan chromatography explored the accuracy of the extraction (saponification) method during the determination process, conducted reproducibility studies on the precision, and established a fast and accurate determination method.

　　Name: Liquid phase silicone column

　　Specification: 250MMX4.6MMX5uM

　　Article number: 2024051003

　　Application:

　　GB/T 17818-1999 Determination of Vitamin D3 in Feed High Performance Liquid Chromatography Method

　　GB/T 17818-1999 Determination of Vitamin D3 in Feed by High Performance Liquid Chromatography

　　GB5009.296-2023 National Food Safety Standard - Determination of Vitamin D in Foods