lucigen 40041-2 40821-4 CloneSmart® HCAmp Blunt Cloning Kit

　　产品简介:北京中北林格供应lucigen 40041-2 40821-4 CloneSmart® HCAmp Blunt Cloning Kit，中北林格是lucigen总代理，提供lucigen产品货号报价查询。其他品牌包括cellscript总代理，Biosearch technologies总代理，epicentre总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。</p>

　　产品名称:lucigen 40041-2 40821-4CloneSmart® HCAmp Blunt Cloning Kit</p>

　　货号:lucigen 40041-2</p>

　　规格:20 rxns</p>

　　存储温度:-20º C</p>

　　产品名称:lucigen 40041-2 40821-4CloneSmart® LCKan Blunt Cloning Kit</p>

　　货号:lucigen 40821-4</p>

　　规格:40 rxns</p>

　　存储温度:-20º C</p>

　　品牌:lucigen</p>

　　供应商:中北林格</p>

　　产地:us</p>

　　发货地:北京</p>

　　lucigen 40041-2 40821-4 CloneSmart® HCAmp Blunt Cloning Kit 产品说明书

　　CloneSmart® Cloning Kits (pSMART® Vectors)

　　CloneSmart®克隆试剂盒（pSMART®载体）

　　产品说明书

　　Convenient, pre-processed vectors eliminate the need for digestion, gel purification, and dephosphorylation.

　　Transcription terminators flanking cloning site stabilize otherwise toxic inserts.

　　Choice of copy number and antibiotic resistance.

　　Optimized for use withE. cloni®10Gelectrocompetentandchemically competentcells (sold separately)

　　方便的预处理载体消除了消化，凝胶纯化和去磷酸化的需要。

　　侧翼克隆位点的转录终止子稳定了有毒的插入物。

　　选择拷贝数和抗生素抗性。

　　优化用于E.cloni®10G电感受态和化学感受态细胞（另售）

　　Clone quickly and facilitate downstream applications

　　Lucigen’s unique cloning kits with pre-processed vectors and highly competent cells eliminate hours of tedious preparation and enable efficient cloning. Ligation can be completed in as little as 30 minutes with no clean-up needed; only a brief heat denaturation is necessary before transformation. Downstream manipulation (e.g, mutagenesis, subsequent insert addition) is facilitated by the minimal vector size (1.7-2.0 kb).

　　快速克隆并促进下游应用

　　Lucigen独特的克隆试剂盒具有预处理载体和高效细胞，可消除繁琐的繁琐制备并实现高效克隆。结扎可在短短30分钟内完成，无需清理;转化前只需要短暂的热变性。通过最小载体大小（1.7-2.0kb）促进下游操作（例如，诱变，随后的插入添加）。

　　Stabilized Inserts and Gap-Free Cloning

　　All cloning kits include the transcription- and translation-free pSMART® vectors (Figure 1), which eliminate many of the problems associated with cloning recalcitrant DNA in conventional plasmid vectors. Conventional cloning vectors contain promoters (e.g., lacZ promoter) that constitutively transcribe the insert sequence. This transcription and coupled translation can destabilize inserts that contain coding regions, strong promoters, short repeats, or incompatible secondary structures. The cloning site of pSMART vectors does not include alacz sequence. In addition, strong transcription terminators flank the cloning site to block spurious transcription from the vector and insert-driven transcription into the vector. Low copy number versions of pSMART further stabilize sequences that are difficult to maintain in typical vectors.

　　稳定插入和无间隙克隆

　　所有克隆试剂盒都包括无转录和翻译的pSMART®载体（图1），它消除了与常规质粒载体中克隆顽固DNA相关的许多问题。常规克隆载体含有组成型转录插入序列的启动子（例如，lacZ启动子）。这种转录和偶联翻译可以使含有编码区，强启动子，短重复或不相容的二级结构的插入物不稳定。pSMART载体的克隆位点不包括lacz序列。此外，强大的转录终止子位于克隆位点的侧翼，以阻止来自载体的杂散转录和插入驱动的转录到载体中。pSMART的低拷贝数版本进一步稳定了在典型载体中难以维持的序列。

　　No Background Problems

　　The pSMART vectors are supplied pre-digested, with blunt, dephosphorylated ends, and are qualified to produce 99.5% recombinant clones in typical experiments. The ultra-low background of empty vector (less than 0.5%) eliminates the need to screen for recombinants, removes the uncertainty of false negatives (light blue pUC colonies) and false positives (white colonies that lack inserts), and enables library construction from nanogram amounts of DNA.

　　没有背景问题

　　pSMART载体预先消化，具有钝的去磷酸化末端，并且在典型实验中有资格产生99.5％重组克隆。空载体的超低背景（小于0.5％）消除了筛选重组体的需要，消除了假阴性（浅蓝色pUC菌落）和假阳性（缺少插入物的白色菌落）的不确定性，并使图书馆构建成为可能。纳克量的DNA。

　　Figure 1.High copy (HC) and low copy (LC) versions of the pSMART transcription-free cloning vectors.

　　图1.pSMART无转录克隆载体的高拷贝（HC）和低拷贝（LC）版本。

　　Convenient Success

　　Efficiently obtain your clone or create your genomic or cDNA library—the CloneSmart Cloning Kits eliminate tedious preparation of vectors and competent cells, as well as time-consuming QC testing experiments. Kits include optimized reagents, ligation-ready vector (no post-ligation cleanup step required), detailed instructions, and trouble-shooting guides to simplify cloning and sequencing. Highly efficientE. cloni® 10Gelectrocompetent cells(up to >4 × 1010cfu/µg) orchemically competent cells( >1 × 109cfu/µg) are available for sale separately.

　　方便成功

　　有效地获得克隆或创建基因组或cDNA文库 - CloneSmart克隆试剂盒消除了繁琐的载体和感受态细胞的制备，以及耗时的QC测试实验。试剂盒包括优化的试剂，准备连接的载体（无需连接后的清理步骤），详细说明和故障排除指南，以简化克隆和测序。高效E.cloni®10G电感受态细胞（最高> 4×1010 cfu /μg）或化学感受态细胞（> 1×109 cfu /μg）可单独出售。

　　lucigen 40041-2 40821-4 CloneSmart® HCAmp Blunt Cloning Kit订购信息

　　Contact your localdistributorfor pricing

　　订购信息

　　CloneSmart Blunt Cloning Kits包含:Vector Premix，CloneSmart DNA连接酶，阳性对照插入DNA，测序引物和完整的方案。试剂盒可与任何大肠杆菌化学感受态或电感受态细胞一起使用，但已经过优化，可与E.cloni®ElectrocompetentCells和Chemically Competent Cells一起使用。

　　对此产品有兴趣，可联系中北林格获取更多详细信息。</p>