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　　Bacillus atrophaeus Nakamura (ATCC® 51189™)

　　Deposited As Bacillus subtilis (Ehrenberg) Cohn

　　Strain Designations 菌株别名 NCTC 10073 [Camp Detrick, DSM 2277, NCIB 8649, NRCB-467]

　　Application 菌株用途 Media testing

　　Sterility testing

　　Testing

　　Ethylene oxide sterilization control

　　Biosafety Level 生物安全等级 1

　　Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

　　Product Format 提供形式 freeze-dried

　　Preceptrol® no

　　Type Strain no

　　Medium 培养基 ATCC® Medium 18: Trypticase Soy Agar/Broth

　　Growth Conditions

　　Temperature 培养温度: 30.0℃

　　Name of Depositor NCTC

　　Chain of Custody ATCC <<--NCTC<<--J.C. Kelsey (Bacillus globigii) <<--- C.R. Phillips

　　References 参考文献 Beeby MM, Whitehouse CE. A bacterial spore test piece for the control of ethylene oxide sterilization. J. Appl. Bacteriol. 28: 349-360, 1965. PubMed: 4954908

　　Kelsey JC. Use of gaseous antimicrobial agents with special reference to ethylene oxide. J. Appl. Bacteriol. 30: 92-100, 1967. PubMed: 6045112

　　Fritze D, Pukall R. Reclassification of bioindicator strains Bacillus subtilis DSM 675 and Bacillus subtilis DSM 2277 as Bacillus atrophaeus. Int. J. Syst. Evol. Microbiol. 51: 35-37, 2001. PubMed: 11211269 Plastics collapsible containers for human blood and blood components--Part 1: Conventional containers. Annex C. Biological tests.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 3826-1:2003.

　　Sterilization of health care products--Biological indicators--Part 2: Biological indicators for ethylene oxide sterilization. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11138-2:1994.

　　Cleanrooms and associated controlled environments--Biocontamination control--Part 1: General principles and methods. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 14698-1:2003.

　　Plastic containers for intravenous injection. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 15747:2003.